한국   대만   중국   일본 
Tes dyagnostik pou SARS-CoV-2 ? Wikipedya Aller au contenu

Tes dyagnostik pou SARS-CoV-2

Depi Wikipedya, ansiklopedi lib

Ou ka jwenn yon tes dyagnostik pou kowonaviris SARS-CoV-2 si gen dout enfeksyon nan Maladi kowonaviris 2019 (Covid-19) sou egzamen klinik ak nan adisyon nan egzamen apwofondi (tomodansitometri, elatriye). Li kapab fet avek tes reyaksyon an chen pa polimeraz apre transcripsyon reves pou deteksyon an ARN viral (RT-PCR) oswa pa tes ki baze sou antiko ELISA pou deteksyon pwoteyin viryon.

Enfrastrikti rechech ewopeyen EVA (Archive de Virus Europeenne) rapidman oganize bay laboratwa dyagnostik atrave mond lan ak eleman viral sevi kom kontwol pou ARN oswa tes ki baze sou pwoteyin. [1]

RT-PCR [ modifye | modifye kod ]

Youn nan premye tes RT-PCR te devlope nan lopital Charite nan Belen nan mwa janvye 2020 ki itilize PCR kantitatif ak fome baz la nan 250 000 twous ke OMS distribye. [2] Sou 2 mas, OMS difize yon pwotokol estanda kreye pa Enstiti Pasteur nan Pari. [3] Dapre yon etid koreyen, tes la gen yon pi ba sansiblite nan deteksyon jen N. [4]

Konpayi Kogenebiotech nan Kore disid te anonse sou 11 fevriye 2020. [5] ke twous deteksyon li yo PowerChek 2019-Ncov™ an tan reyel PCR Twous, ki vize jen "E" pataje pa tout Betacoronavirus ak jen RdRp espesifik nan SARS-CoV-2, [6] te resevwa yon otorizasyon pou itilize an ijans nan men otorite koreyen ak ameriken yo.

Lot konpayi tankou Seegene [7] ak Solgent te devlope tou nan mwa fevriye 2020 vesyon yo twous deteksyon nan kalite klinik (bon jan kalite yo) ki rele "DiaPlexQ" ak "Allplex 2019-nCoV assay" respektivman.

Nan Etazini, Centers for Disease Control distribye tes dyagnostik CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR pou laboratwa sante piblik. [8] Nan premye vesyon yo, youn nan twa tes jenetik te bay rezilta doute CDC Atlanta; tes pi resan yo le yo sevi avek de (2) konpozan yo te jije serye sou 28 fevriye 2020, sa ki pemet laboratwa nasyonal ak lokal yo byen vit konplete tes yo. [9]  : Tes sa a te apwouve pa Food and Drug Administration (FDA) anba yon otorizasyon pou itilize an ijans.

Inivesite Syans ak Teknoloji nan Hong Kong pwopoze nan komansman mwa fevriye yon tes RT-PCR mikwoflyidik tre rapid. [10]

Laboratwa referans [ modifye | modifye kod ]

Sou 4 mas 2020, OMS kreye yon lis laboratwa referans ofisyel yo ak pwotokol devlopman [11]  :

Peyi Enstiti Sib jenetik
Drapo Almay Almay Charite RdRp, E, N
Drapo Repiblik Pèp la nan Lachin Chin CDC chinwa ORF 1ab ak nucleoprotein (N)
Drapo Etazini Etazini CDC ameriken Twa (3) sib nan jen N
Drapo Lafrans Frans Enstiti Pasteur, Pari De (2) sib nan jen RdRp
Drapo Hong Kong Hong Kong HKU ORF1b-nsp14, N
Drapo Japon Japon Enstiti Nasyonal pou Maladi Enfeksyon Pwoteyin Spike (Peplomer)
Drapo Taylann Taylann Enstiti Nasyonal Sante N

Antiko [ modifye | modifye kod ]

COVID-19 kapab dyagnostike tou gras a twous deteksyon antiko. Tes iminolojik yo itilize yon echantiyon nan serom san epi yo ka bay yon rezilta pozitif menmsi moun nan refe epi viris la pa prezan anko. Premye tes iminolojik la te dekri pa yon ekip nan Enstiti viroloji nan Wuhan sou 17 fevriye 2020. [12] , [13]

Sou 25 fevriye, yon ekip nan lekol "Ecole de medecine Duke ? NUS" nan Sengapou te anonse yon lot tes antiko pou COVID-19 ki ta ka bay rezilta nan kek jou. [13] , [14]

Sou 28 fevriye 2020, yon lot konpayi Kore disid ki rele PCL aplike pou deziyasyon akselere Ministe Sekirite alimante ak medikaman Kore disid pou twous deteksyon ki baze sou antiko li, " COVID-19 Ag. GICA Rapid ". Kontreman ak yon twous deteksyon ki baze sou RT-PCR an tan reyel, PCL afime ke twous li ki baze sou antiko te kapab fe dyagnostik nan 10 minit.

Asid nikleyik [ modifye | modifye kod ]

Tes "Explify Respiratory" nan IDbyDNA se yon tes analiz metajenomik ki ka idantifye plis pase 900 ajan patojen respiratwa, ki gen ladan SARS-CoV-2. Analiz done reyel pran mwens pase yon edtan; peryod ant resepsyon echantiyon an ak rezilta tes la se 36 edtan. Li pi che pase tes RT-PCR. [15]

Pwodiksyon twous tes yo [ modifye | modifye kod ]

Lachin te anonse ke li fe 1,7 milyon tes asid nikleyik ak 350 000 twous tes antiko chak jou sou 25 fevriye 2020. [16]

Kolekte echantiyon [ modifye | modifye kod ]

Tes RT-PCR an tan reyel ka fet sou echantiyon respiratwa yo te jwenn nan dives metod, ki gen ladan yon prelevman nazofarenje oswa yon echantiyon krache. [17] , [18] Rezilta yo anjeneral disponib nan kek edtan a 2 jou. [19]

Hong Kong mete an plas yon sistem pou pasyan sispek yo rete nan kay la: sevis ijans la bay yon tib pou echantiyon pasyan an. Pasyan an krache nan tib epi retounen li. Pasyan an resevwa yon rezilta tes kek tan apre. [20]

NHS britanik la te anonse tou ke li pral komanse fe tes ka sispek nan kay la, ki evite risk pou yo enfekte lot moun nan lopital la ak gen dezenfekte anbilans lan si yo itilize. [21]

Skane pilmone [ modifye | modifye kod ]

Skane pilmonese yon zouti imajri woutin pou dyagnostik pou nemoni, rapid epi relativman fasil nan adisyon a egzamen klinik, san yo yon tes deteksyon viral pou pale egzak. Yon rechech revele ke sansiblite metod la pou deteksyon COVID-19 te 98%, konpare ak yon sansiblite nan 71% pou RT-PCR. [22] Pou etid sa a, cheche nan Lopital Tongji nan Wuhan, nan Chin, komanse etidye vale dyagnostik la ak amoni nan imajri pwatrin konpare ak tes RT-PCR pou dyagnostik COVID-19.

Lye ak kondisyon preskripsyon tes [ modifye | modifye kod ]

Nan Lafrans, pou egzanp, deteksyon se fe nan lopital. [23] Dire a se relativman lontan paske li nesese voye echantiyon yo nan youn nan 45 sant deteksyon yo [24] epi egzekite tes la ki kounye a pran 1/2 jou. Pou limite gaye, li ta san dout dwe preferab pou medsin vil yo gen mwayen mobil [25] , [26] nan deteksyon, ki ta ka ogmante vites depistaj ak jeneralize deteksyon menm anvan aparisyon siy klinik yo. Vreman vre, pousantaj moun ki enfekte men san senptom ta dwe ant 1 ak 50%, selon sous, [27] , [28] , [29] ak an patikilye nan popilasyon an jivenil ki ta ka enfekte otan ke popilasyon granmoun. [30] Sepandan, Ministe Sante a [31] pa prevwa yon pwotokol deteksyon konsa sistematik, paske li pa gen bidje korespondan. [32] , [33] , [34] Egzanp kontaminasyon nan mezon retret Vill'alize [35] montre danje pou repote aplikasyon yon tes depistaj sou faz sentom maladi a. Pa fason pou konparezon, an 2009, Eta a te depanse 2.2 milya ewo pou vaksinasyon kont grip A (H1N1) pou yon epidemi ak 10 fwa [36] mwens risk pou yo mouri. Kantite tes depistaj nan Frans se vreman ba konpare ak 1.7 milyon tes pratike nan peyi Lachin : pa relasyon chif sa a ak popilasyon an nou we ke tes kotidyen yo omwen 45 [37] fwa pi wo nan peyi Lachin. Dapre Imperial College de Londres li nesese pou bloke "plis pase 60% kontaminasyon pa mezi siveyans pou kontwole epidemi an". [38]

Referans [ modifye | modifye kod ]

  1. ↑ ( angle ) en ≪ Wuhan coronavirus | EVAg ≫ . european-virus-archive.com . Archived from the original on 2020-03-04 . Retrieved 4 mas 2020 .  
  2. ↑ ( angle ) en Cormac Sheridan (19 fevriye 2020). ≪ Coronavirus and the race to distribute reliable diagnostics ≫ . Nature Biotechnology : d41587?020?00002-2. ISSN   1087-0156 . doi : 10.1038/d41587-020-00002-2 . Retrieved 3 mas 2020 .  
  3. ↑ ( angle ) en Institut Pasteur, Paris (2 mas 2020). ≪ Protocol: Real-time RT-PCR assays for the detection of SARS-CoV-2 ≫ (pdf) . OMS.  
  4. ↑ ( angle ) en "27/2: Insufficient Sensitivity of RNA Dependent RNA Polymerase Gene of SARS-CoV-2 Viral Genome as Confirmatory Test using Korean COVID-19 Cases"
  5. ↑ ( angle ) en ≪ The 1st kit 'PowerChek™ 2019-nCoV Real-time PCR Kit' for EUAL by KFDA & KCDC ≫ . Kogene. 11 fevriye 2020. Archived from the original on 2020-04-08.  
  6. ↑ ( angle ) en ≪ [NEW PRODUCT] COVID-19 KIT ≫ . Kogene. 27 fevriye 2020. Archived from the original on 2020-04-23.  
  7. ↑ ( angle ) en Seegene - Sitweb ofisyell
  8. ↑ ( angle ) en ≪ Information about Emergency Use Authorization for 2019 Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel ≫ . CDC. 13 fevriye 2020.  
  9. ↑ ( angle ) en ≪ Transcript for the CDC Telebriefing Update on COVID-19 ≫ . CDC. 8 fevriye 2020.  
  10. ↑ ( angle ) en ≪ HKUST Research Team Invents World’s Fastest Coronavirus Detection Device Offering Diagnostic Results in 40 Minutes ≫ . The Hong Kong University of Science and Technology . Retrieved 4 mas 2020 .  
  11. ↑ ( angle ) en ≪ Laboratory guidance ≫ . OMS . Retrieved 4 mas 2020 .  
  12. ↑ ( angle ) en Zhang, Wei; Du, Rong-Hui; Li, Bei; Zheng, Xiao-Shuang (1 janvye 2020). ≪ Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes ≫. Emerging Microbes & Infections . p. 386?389. doi : 10.1080/22221751.2020.1729071 .  
  13. ↑ 13,0 et 13,1 ( angle ) en Normile Dennis (27 fevriye 2020). ≪ Singapore claims first use of antibody test to track coronavirus infections ≫ . sciencemag.org . Retrieved 2 mas 2020 .  
  14. ↑ ( angle ) en ≪ Duke-NUS used COVID-19 antibody tests to establish link between church clusters in a world-first ≫ . channelnewsasia.com . Archived from the original on 2020-03-02 . Retrieved 2 mas 2020 .  
  15. ↑ ( angle ) en Sheridan, Cormac (19 fevriye 2020). ≪ Coronavirus and the race to distribute reliable diagnostics ≫ . Nature Biotechnology : d41587?020?00002-2. ISSN   1087-0156 . doi : 10.1038/d41587-020-00002-2 . Retrieved 4 mas 2020 .  
  16. ↑ ( angle ) en Emmi Laine (25 fevriye 2020). ≪ China Makes Over 1.7 Million Covid-19 Testing Kits per Day, Official Says ≫ . Yicai Global.  
  17. ↑ ≪ 2019 Novel Coronavirus (2019-nCoV) Situation Summary ≫ . Centers for Disease Control and Prevention. 30 janvye 2020. Archived from the original on 26 janvye 2020 . Retrieved 30 janvye 2020 .  
  18. ↑ ( angle ) en ≪ Real-Time RT-PCR Panel for Detection 2019-nCoV ≫ . Centers for Disease Control and Prevention. 29 janvye 2020. Archived from the original on 30 janvye 2020 . Retrieved 1 fevriye 2020 .  
  19. ↑ ( angle ) en ≪ Curetis Group Company Ares Genetics and BGI Group Collaborate to Offer Next-Generation Sequencing and PCR-based Coronavirus (2019-nCoV) Testing in Europe ≫ . GlobeNewswire News Room. 30 janvye 2020. Archived from the original on 31 janvye 2020 . Retrieved 1 fevriye 2020 .  
  20. ↑ ( angle ) en ≪ In Age of COVID-19, Hong Kong Innovates To Test And Quarantine Thousands ≫ . NPR.org . 23 fevriye 2020.  
  21. ↑ ( angle ) en ≪ NHS pilots home testing for coronavirus ≫ . mobihealthnews.com . 24 fevriye 2020.  
  22. ↑ ( angle ) en ≪ CT provides best diagnosis for COVID-19 ≫ . Science Daily. 26 fevriye 2020.  
  23. ↑ "Ouest- France:Coronavirus. A Rennes, quatre heures pour detecter (ou pas) le virus"
  24. ↑ ( franse ) fr "Tf1, 5 Mars: Coronavirus : pas de tests systematiques dans l'Hexagone"
  25. ↑ "Journal le Point, 7/2: Coronavirus : l'appareil qui peut revolutionner sa detection"
  26. ↑ ( angle ) en "RealStar® SARS-CoV-2 RT-PCR Kit RUO"
  27. ↑ "Science lart, 24/2: A Person Can Carry And Transmit COVID-19 Without Showing Symptoms, Scientists Confirm "
  28. ↑ "Hufington post, 28/2/20:Ces 4 cas de coronavirus qui interpellent les chercheurs"
  29. ↑ "MedRixv: Estimation of the asymptomatic ratio of novel coronavirus infections (COVID-19)"
  30. ↑ "Nature, 5/3/20:Coronavirus latest: children are as susceptible as adults, study suggests"
  31. ↑ "Tf1,f1, 6/3/20:Tests de depistage du coronavirus : comment fonctionnent-ils en France ?"
  32. ↑ "Journal Liberation, 5/3/20:A qui propose-t-on actuellement des tests de diagnostic du Covid-19 ? "
  33. ↑ "le temps, 27/2/20: Coronavirus: qui va payer les tests de depistage? "
  34. ↑ "6/3/20,new observer:NC governor emphasizes need for more coronavirus test kits "
  35. ↑ "7/3/20, ma commune:Coronavirus : 10 personnes agees confinees a l'Ehpad de Thise"
  36. ↑ "Nice matin: SRAS, peste, ebola, grippe H1N1... le coronavirus est-il plus dangereux que les autres grandes epidemies?"
  37. ↑ ( franse ) fr "Journal france 2, 6/3/20: 7min"
  38. ↑ ( franse ) fr Incubation Contagiosite Mortalite Les mots-qui comptent pour decrire le coronavirus

Lyen deyo [ modifye | modifye kod ]

Rekipere depi ≪  https://ht.wikipedia.org/w/index.php?title=Tes_dyagnostik_pou_SARS-CoV-2&oldid=820449  ≫