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	weisslab .ph .biu .ac .il

????? ???? (???? ?- 20 ???? 1956 ) ??? ??????? ? ????? ??????? ??????????? ??-???? , ??? ?? , ????? . ??? ??????? ? ????? , ???????? ? ?????????? ? ?????????? ????????? ???? ???'?? , ????? ????? . ?????? ?????? ???? ???? ? ????????? ?? ???????? ?????? .

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????? ???? ???? ? ???? , ????? . ??? ???? ?? ??????? ? ?????? - ???? ???????? ?????? ???? 1989 ????? ?? ???? ????? ??????? ??? ? ?????? ????? ?? ????? 1989 ?-1990. ????? 1990 ?? 2001 ??? ??? ??? ? ????? ??????? ????? ????? ??????? ?????. ???? 2001 ??? ??????? ? ????? , ???????? ? ?????????? ? ?????????? ????????? ???? ???'?? ???? ???? 2011 ??? ??????? ?? ?????? . ???? 2015 ??? ???? ???????? ? ?????? ??????????? ??-????. ????? ???? ?? ??? ??????? ???? ? ???? ????? ??????? ????? 2007 ?? 2011, ??????? ???? ??????? ?????? ????????? ????????? ? ???? ????? ???? ????? 2007 ?? 2015, ???? ???? ?????? ??????? ??????? ? ???? ????????? ?? ????????? ????? 2010 ?-2014. ??? ??, ??? ??????? ???? ? ???-????? ? ?????????? ??????? ???? 2010, ??????? ???? ??????? ? ?????????? ?????? ????? 2013 ?? 2018 ????? ???? ????? ???????? ??????? ?? ?????????? ????? .

????? ???? [ ????? ??? ???? | ????? ]

????? ?? ????? ???? ????? ???? ? ????????? ?? ???????? ?????? ?? ??? ???-????????? , ?????? ???? ???????. ????????? ?? ???????? ?????? ????? ???? ??????? ????????????? ?? ???-???????? ??????. ?????? ?????? ????????????? ??????????, ??? ?????? ????? ?? ???????? ???????? ?????? ???????, ????????? ?? ???????? ?????? ?????? ????? ??????? ?? ?? ??????? ????? ??????? ???? ?? ?? ????????? ??? ??? ???? ????? ????. ???? ?? ????? ??????? ????? ?? ????? ????? ?? ?? ??? ???? ????? ????? ??? ????? ????? ???????? ???????? ??????? ???? ???????? ???? ??????? ??????? ? ?????? ?? ???????? . ?????? ?? ????? ???? ?????? ?????? ?? ???? ?????? ???? ?????? ?? ?????? ???? ???????? ??????. ??? ?????? ????? ?????? ???? ???? ?????? ?????? ???????????? ????????? ?????? (smFRET), ????? ?? ?????? ???????? ??????, ?????? ?? ??????? ??????? ?????? ??? ????? ????? ??????? ??????, ????????? ??? ????? ??????? ????? ?????-???. ?????? ?? ????? ???? ?? ????? ?? ??????? ????????? ?? ??????? ??????? ???? ??? ?????, ??? ????? ?????? ????????? ?????.

?????? ??????? [ ????? ??? ???? | ????? ]

?????? ?? ????? ???? ????? ??????? ???? ??????? ?? ???? ???? ?????? ?????? ???????????? ????????? ?????? (smFRET). ^[1] ????? ?????? ????? ?? ???????? DNA ?????? ?? ???? ^[2] ????? ??? ?????? ????? ?? ???? ???????? ? ????? . ^[3] ????? ???? ?? ?????? ?????? ?? ????? ???? ????? ????? ??? ?? ?????? ???? ?????? ?? ????? ??????? ????????? ?????? ^[4]^[5] ??????? ???????? ?????? ????? ?? ??? ? ????? RNA ???????? . ^[6] ???? ?? ????? ?? ??? ?????? ???? ???? ??? ??????.

?????? ?? ????? ???? ????? ?? ??????? ?????? ? ?????? ???????? ???? ??????? ? ???????? . ?????? ????? ?? ?????? ?? ??????? ?????????? ? ?????????? ????????? ?????? ?? ????? ????????? ?? ????? ??????? ???????? ?????? ?????????? ???????? ????????? ^[7] ??? ????? ?? ????? Quantum Dot Corp ??? ????? ? ???? ???? ????????? . ????? ??????? ???????? ????? ?? ????? ?? ???? ???? ???? ?????? ?????? ???????? ?????????????. ?? ????? ????, ?????? ????, ????? ?????? ?? ??????? ? ????? ????. ?????? ?? ???? ?? ?????? ??????? ???????? ?????? ?? ?????? ??? ????????? ?????? ??? ????? ???? ?????? ?????. ^[8] ??????? ????????? ????? ????? ????? ? ????? ????????? ?? ???????? ??????? ??? ????? ?????.

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^ Ha T, Enderle T, Ogletree D, Chemla D, Selvin P, Weiss S. Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. Proceedings of the National Academy of Sciences. 1996; 93(13):6264-6268.
^ Ha T, Ting A, Liang J, Deniz A, Chemla D, Schultz P, Weiss S., “Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism”, Proceedings of the National Academy of Sciences 1999, 96 (3), 893-898.
^ Deniz, A. A.; Dahan, M.; Ha, T.; Grunwell, J.; Faulhaber, A.; Chemla, D. S.; Weiss, S.; Schultz, P. G., “Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Forster distance dependence and subpopulations”, Proc. Natl. Acad. Sci. USA 1999, 96, 3670-3675.
^ Deniz, A.A., Laurence, T.A., Beligere, G.S., Dahan, M., Martin, A.B., Chemla, D.S., Dawson, P.E., Schultz, P.G., and Weiss S., Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2. Proc Natl Acad Sci U S A 2000, 97, (10), 5179-84.
^ Michalet, X.; Weiss, S.; Jager, M.; “Single-molecule fluorescence studies of protein folding and conformational dynamics”, Chemical reviews, 2006, 106 (5), 1785
^ Kapanidis A, Margeat E, Ho S, Kortkhonjia E, Weiss S, Ebright R. Initial Transcription by RNA Polymerase Proceeds Through a DNA-Scrunching Mechanism. Science. 2006; 314(5802):1144- 1147.
^ Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos A. Semiconductor nanocrystals as fluorescent biological labels. Science. 1998; 281(5385):2013-2016.
^ Park K, Weiss S. Design Rules for Membrane-Embedded Voltage-Sensing Nanoparticles. Biophysical Journal, 2017 112(4), 703-713.

[1] Ha T, Enderle T, Ogletree D, Chemla D, Selvin P, Weiss S. Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. Proceedings of the National Academy of Sciences. 1996; 93(13):6264-6268.

[2] Ha T, Ting A, Liang J, Deniz A, Chemla D, Schultz P, Weiss S., “Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism”, Proceedings of the National Academy of Sciences 1999, 96 (3), 893-898.

[3] Deniz, A. A.; Dahan, M.; Ha, T.; Grunwell, J.; Faulhaber, A.; Chemla, D. S.; Weiss, S.; Schultz, P. G., “Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Forster distance dependence and subpopulations”, Proc. Natl. Acad. Sci. USA 1999, 96, 3670-3675.

[4] Deniz, A.A., Laurence, T.A., Beligere, G.S., Dahan, M., Martin, A.B., Chemla, D.S., Dawson, P.E., Schultz, P.G., and Weiss S., Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2. Proc Natl Acad Sci U S A 2000, 97, (10), 5179-84.

[5] Michalet, X.; Weiss, S.; Jager, M.; “Single-molecule fluorescence studies of protein folding and conformational dynamics”, Chemical reviews, 2006, 106 (5), 1785

[6] Kapanidis A, Margeat E, Ho S, Kortkhonjia E, Weiss S, Ebright R. Initial Transcription by RNA Polymerase Proceeds Through a DNA-Scrunching Mechanism. Science. 2006; 314(5802):1144- 1147.

[7] Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos A. Semiconductor nanocrystals as fluorescent biological labels. Science. 1998; 281(5385):2013-2016.

[8] Park K, Weiss S. Design Rules for Membrane-Embedded Voltage-Sensing Nanoparticles. Biophysical Journal, 2017 112(4), 703-713.

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