Physical containment of pathogenic organisms or agents in microbiology laboratories
One use of the concept of
biocontainment
is related to
laboratory biosafety
and pertains to
microbiology
laboratories in which the physical containment of
pathogenic
organisms or agents (
bacteria
,
viruses
, and
toxins
) is required, usually by isolation in environmentally and biologically secure cabinets or rooms, to prevent accidental infection of workers or release into the surrounding community during scientific research.
Another use of the term relates to facilities for the study of agricultural pathogens, where it is used similarly to the term "
biosafety
", relating to safety practices and procedures used to prevent unintended infection of plants or animals or the release of high-consequence pathogenic agents into the environment (air, soil, or water).
Terminology
[
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]
The
World Health Organization
's 2006 publication,
Biorisk management: Laboratory biosecurity guidance
, defines laboratory biosafety as "the containment principles, technologies and practices that are implemented to prevent the unintentional exposure to pathogens and toxins, or their accidental release". It defines
biorisk
management as "the analysis of ways and development of strategies to minimize the likelihood of the occurrence of biorisks".
[1]
The term "biocontainment" is related to laboratory biosafety.
[2]
[3]
Merriam-Webster
's online dictionary reports the first use of the term in 1966, defined as "the containment of extremely pathogenic organisms (such as viruses) usually by isolation in secure facilities to prevent their accidental release especially during research".
[4]
The term laboratory biosafety refers to the measures taken "to reduce the risk of accidental release of or exposure to infectious disease agents", whereas laboratory biosecurity is usually taken to mean "a set of systems and practices employed in legitimate bioscience facilities to reduce the risk that dangerous biological agents will be stolen and used maliciously".
[5]
Containment types
[
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]
Laboratory context
[
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]
Primary containment
is the first container in direct contact with biohazardous material
[6]
as well as protection of personnel and the immediate laboratory environment from exposure to infectious agents. Primary containment requires using proper storage containers, good microbiological technique, and the use of appropriate safety equipment such as biological safety cabinets.
Secondary containment
is the protection of the environment external to the laboratory from exposure to infectious materials and is provided by a combination of facility design and operational practices.
Biological safety cabinets
(BSC), first commercially available in 1950,
[7]
are fairly common devices designed to provide effective primary biocontainment in laboratories working with highly infectious agents. Three general levels and types have been devised (
Class I, Class II, and Class III
).
Biosafety suites are suites of laboratory rooms which are essentially equivalent to large Class III cabinets in which
positive pressure personnel suits
("space suits") serve as the "outside" environment for workers. Examples include the biosafety suites at
USAMRIID
at
Fort Detrick, Maryland
, USA and the
Maximum Containment Facility
(MCF) of the
CDC
in
Atlanta, Georgia
, USA.
Agricultural context
[
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]
The term “biocontainment” is used differently in facilities for the study of human pathogens versus those used for the study of agricultural pathogens. In agricultural facilities, the definition for “biocontainment” resembles that for “biosafety,” i.e., the safety practices and procedures used to prevent unintended infection of plants or animals or the release of high-consequence pathogenic agents into the environment (air, soil, or water). In the agricultural setting, worker protection and public health are always considerations; however, emphasis is placed on reducing the risk that agents under study could escape into the environment.
Biosafety levels
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]
A "biosafety level" (BSL) is the level of the biocontainment precautions required to isolate dangerous
biological agents
in an enclosed laboratory facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4). In the United States, the
Centers for Disease Control and Prevention
(CDC) have specified these levels.
[8]
In the
European Union
, the same biosafety levels are defined in a
directive
.
[9]
Guidelines
[
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]
Today, guiding publications for biosafety and containment in the US are set by the
Centers for Disease Control and Prevention
(CDC) and the
National Institutes of Health
(NIH).
[10]
Since 1984, the CDC and the NIH have jointly authored the
Biosafety in Microbiological and Medical Laboratories
(
BMBL
). The
BMBL
is an advisory document providing national recommedations for
Biosafety Levels
, Containment,
Decontamination
and
Disinfection
, Transportation, and Disposal of biohazardous agents.
[10]
In Canada the government publication "Laboratory biosafety guidelines" was current between 1990 and 2013,
[11]
[12]
and has been superseded by the "Canadian Biosafety Standards and Guidelines".
[13]
[14]
OECD Best Practice Guidelines for Biological Resource Centres
is a consensus report created in 2001 after experts from
OECD
countries came together, calling upon "national governments to undertake actions to bring the BRC concept into being in concert with the international scientific community". BRCs are "repositories and providers of high-quality biological materials and information".
[15]
Laboratory program
[
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]
Components of a laboratory biosecurity program include:
[5]
See also
[
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]
- Aeromedical Isolation Team
? Former US Army aeromobile biocontainment team
- Biorisk
? Risk associated with biological materials and/or infectious agents ("pathogens")
- Biosafety
? Prevention of large-scale loss of biological integrity
- Biosafety level
? Set of biocontainment precautions
- Biosecurity
? Preventive measures designed to reduce the risk of infectious disease transmission
- Biological hazard
? Biological material that poses serious risks to the health of living organisms
- Chemical hazard
? Non-biological hazards of hazardous materials
- Safety engineering
? Engineering discipline which assures that engineered systems provide acceptable levels of safety
- Security engineering
? Process of incorporating security controls into an information system
- Select agent
? Controlled biological agents in the United States
References
[
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]
Citations
- ^
"Biorisk management: Laboratory biosecurity guidance"
(PDF)
. World Health Organization. September 2006
. Retrieved
23 May
2020
.
- ^
"Biosafety"
.
US Dept of Health & Human Services. Public Health Emergency (Science Safety Security)
. Retrieved
22 May
2020
.
- ^
"Biocontainment"
.
US Dept of Health & Human Services. Public Health Emergency (Science Safety Security)
. Retrieved
22 May
2020
.
- ^
"Definition of biocontainment"
.
Merriam-Webster: Dictionary
. 18 March 2020
. Retrieved
23 May
2020
.
- ^
a
b
Salerno, Reynolds M.; Gaudioso, Jennifer; Brodsky, Benjamin H. (2007).
"Preface"
.
Laboratory Biosecurity Handbook
(Illustrated ed.). CRC Press. p. xi.
ISBN
9781420006209
. Retrieved
23 May
2020
.
- ^
Definition of primary containment
- ^
Wedum, A.G. (1969), "The Detrick experience as a guide to the probable efficacy of P4 microbiological containment facilities for studies on microbial recombinant DNA molecules";
J Am Biol Safety Assoc
;1:7-25.
- ^
Richmond JY, McKinney RW (editors) (1999).
Biosafety in Microbiological and Biomedical Laboratories
(4th ed.).
ISBN
0-7881-8513-6
.
- ^
Council Directive 90/679/EEC of 26 November 1990 on the protection of workers from risks related to exposure to biological agents at work, OJ No. L 374, p. 1.
- ^
a
b
Meechan, Paul J; Potts, Jeffrey (2020),
Biosafety in Microbiological and Biomedical Laboratories (BMBL)
(PDF)
(6th ed.), Centers for Disease Control and Prevention
- ^
"Animal Pathogen Containment Levels".
Containment Standards for Veterinary Facilities Sections 1-3
. Canadian Food Inspection Agency. 2013-08-01. Archived from
the original
on 17 June 2013.
- ^
"Laboratory biosafety guidelines / Published by authority of the ... Centre for Emergency Preparedness and Response"
. Public Works and Government Services Canada. 2013-04-03.
- ^
"Facilities Handling Animal Pathogens"
. Canadian Food Inspection Agency. 2013-08-01.
- ^
"Canadian Biosafety Standards and Guidelines"
. Public Health Agency of Canada. 2022-11-24.
- ^
"OECD Best Practice Guidelines for Biological Resource Centres"
.
OECD
. Retrieved
23 May
2020
.
pdf
Other sources
- Biosafety in Microbiological and Biomedical Laboratories
(1999), 4th Edition,
U.S. Department of Health and Human Services
,
Public Health Service
,
Centers for Disease Control and Prevention
,
National Institutes of Health
,
Washington, DC
:
U.S. Government Printing Office
.
- The 2013 International Conference on Biocontainment Facilities
- The 2014 International Conference on Biocontainment Facilities
- eBook Reference: Management Principles for Building and Operating Biocontainment Facilities (Kindle Edition)
Archived
2013-05-26 at the
Wayback Machine
- Wedum, A.G., W.E. Barkley, and A. Hellman (1972), "Handling of infectious agents",
Journal of the American Veterinary Medical Association
, 161(11):1557-1567.
Further reading
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