?????? ??? ?????????
??
???? ?????????????
???
??? ????
???? ?? ????? ?????? ????? ???? ??
?????? ???????
???? ???? ????????? ??? ??? ????
????? ????????
?? ?????? ???
????? ?????
.
???? ???????
???? ??10
???????
??? 400 ????????
???????
???? ?? 3 ??? 124
??????? ????
.
????? ???? ??? ????????? ?? ???? ?????? ?????? ??????
?????? ???????
??
????? ??????
. ???? ??
???? ?????
(?? ????
?????????
??
??????
) ??? ???? ?????? ????????? ????? ?????? ?? ?????? ?????? ??
?????
. ??? ???? ?????? ?? ????? ????? ?????? ??? ????????? ??? ????? ????? ????? ??
???? ?????
? ???? ??? ??? ?????? ??? ??????? ???? ?? ???? ????? ?? ???? ???? ?????.
????????
[
???
]
??? ?????? ?????? ??? ????????? ?????? ??????? ????? ???
????? ?????
???? ????? ???? ??? ??????? ???? ?????. ??? ???
1801
????
?????????
????????
??? ?????? ????
(
??????????
:
Johann Wilhelm Ritter
) ?? ???? ??? ?????? ??? ?????? ???? ?? ????? ???????? -???? ?? ????? ????? ??????-? ????? ???? ??? ?? ????? ????? ??? ??? ????? ??????
?????????
???? ???????? ≪?????? ????????≫ ????? ???
??????? ?????????
????????? ?? ≪?????? ??????≫ ???? ?? ?????? ????? ?? ?????. ?? ?????? ????? ≪?????? ??????????≫ ??? ??? ????? ????? ???? ??? ????? ??? ????????? ????
????? ?????? ???
. ?? ????? ????? ??? ?? ????????? ????????? ???? ???????? ????? ???? ??????? ????????? ?????? ??? ?????????
??????? ??? ???????
??? ???????.
[1]
???? ?????? ??? ??????? ?? ??? 200 ??????? ????????? ??? ?????? ?????? ????? ??? ??????? ????????? ???????? ?????? ????? ??? 1893.
[2]
???? ???????
[
???
]
???? ??????? ??????? ?????? ??? ????? ???????? (Ultra Violet). ??? ?? ?????? ??? ??????? ?? ???? ?? ????????? ??? ???? ??? ?????.
????? ?????? ??? ?????????
[
???
]
???? ?????? ??? ????????? ??? ??? ????? ??????? ?? ????? ????? ??? ??????? ??? ????? ????? ???? (ISO-DIS-21348) ?? ????? ????????? ???????:
[3]
??? ??????
|
?????
|
??? ??????
????????
|
????
??????
???
???? ?????
|
???? ??? ??????? ?????
??
????? ??????
|
UVA
|
400 ?.??320 ?.?
|
3.10?3.94
eV
|
?????? ???????
|
NUV
|
400 ?.??300 ?.?
|
3.10?4.13 eV
|
?????? ???????? ?? ???? B
|
UVB
|
320 ?.??280 ?.?
|
3.94?4.43 eV
|
Middle
|
MUV
|
300 ?.??200 ?.?
|
4.13?6.20 eV
|
?????? ??????? ?? ???? C
|
UVC
|
280 ?.??100 ?.?
|
4.43?12.4 eV
|
Far
|
FUV
|
200 ?.??122 ?.?
|
6.20?10.2 eV
|
?????? Vacuum
|
VUV
|
200 ?.??10 ?.?
|
6.20?124 eV
|
???? Extreme
|
EUV
|
121 ?.??10 ?.?
|
10.2?124 eV
|
??????
??????? ???????
(photolithography)
???????
?????? ???? ??? ??????? ????? (DUV ?? Deep UV) ???
?????? ???????
???? ??? ?? 300 ? ?. ???? ?????? ???????? ???? ????? ???
??????
?????? ????? ??? ???????????? ???? ??????? ???. ?? ?????? ?????? ?? ??? 150?200 ? ? ???
???????
?? ?????? ????? ???? ???? ??? ??????? ???????? ??? ????????? ???????? ???? ????? ???? ??????? ??? ?? ??? ?? ?? ???? ????? ?? ?????????? ??????? ????
??????????
????? ???? ??? ??? ????? ?????? ??? ??? ??????.
????? ??????
[
???
]
????? ??????
?? ????? ??? (???? ??? ??????
????? ?????? ???
)? ?? ????? ???? ???? ??? ??????? ????? ????? ?? ????? ??????. ??? ????? ?????? ???? ≪???? ??? ??????? ?????≫ (
???????????
:
UV light
)?. ??? ??????? ???????? ??????? ???? ????? ??????? ???????? ??????? ???? ??? ???? ??????
????????
??? ????? ??????? ??????? ?????? ????? ????? ???? ?????? ???? ???? ?????
???? ???
? ??? ???? ???? ?????? ?????? ??? ???? ?? ??? ???? ?? ??? ???? ???? ?? 400 ???????. ?????? ??? ???????? ??? ????? ??? ≪??? ???? ?? ????≫ ?? "blacklight blue" ?? ???????? "BLB" ???????? ?? ?????? ????? ????? ??????? ("bug zapper" blacklight "BL") ????? ?? ????? ??? ??? ???? ??? ??????. ???????? ???????? ?????? ??????? ??? ?????? ???? 368 ??? 371 ?.? ??? ?? ???? ????? ?????????
?????????
????
?????????
(SrB4O7F:Eu2+) ?? ????? ??????????? (SrB4O7:Eu2+) ????? ?????? ???????? ?????? ????? ???? 350 ??? 353 ?.? ??? ?????? ???????? ??????? ??????? (BaSi2O5:Pb+). ?????? ????? ?????? ??????? ?? 365 ?.?.
???? ????? ?????? ????? ?? ???? ???? ??? ?????????? ?????? ????? ??? ??? ?????? ??????? "UVA". ??? ?????? ???? ??? ??????? UVB ?UVC? ?????? ???? ??????? ???? ????? ?????? ?????
DNA
????? ??? ???????
?????? ?????
. ????? ?????? ?? ??????? ?????? ??????? ??? ???????? ???????? ??? ?? ???? ????? ?????? ???? ??????? ??????? ??? ????? ??? ???????
?????? ?????????
??????
??????? ???
??????? ??????.
????? ????? ????? ?????? ?? ???? ??????? ?????? ??????
????? ???
????? ??????? ??????. ?????? ??? ??? ???? ?????? ????? ??????. ??? ??? ?????? ?????? ?????? ?????????? ????? ?????? ????????? ??? ???? ?????? ????? ??? ??????? (??? ?? 0.1% ?? ?????? ???????) ???? ?????? ????? ?????? ?? ???? ??????? ??????. ??????? ??? ????????? ??????? ????? ??? ???????? ??? ???? ??? ??????? ???? ??????? ???? ?????????. ??? ?????? ????? ???????? ??????? ?? ???? ?????? ??? ???? ????? (???? ??
?????
)
??????? ?????? ??? ????????? ??????? ???????? ????? ????? ????? ???. ??? ???????? ?????? ???? ????? ?????? ??? ??????? ???????? ????????? ?????? ???? ???? ??? ???? ?????????.
??? ?????? ????????? ??? ????????? ???? ????? ???? ???? ??????? ?????? ??? ?????? ????? ??????? ??????? ??? ???????? ???????? ?????? ??????? ?? ???????? ???????? ?????? ?????? ???? ???? ???? ?? ???? ??? ?????? ??????. ??????? ?????? ??? ???? ????????? ??? ?????? ???????? ??? ???? ???? ???? ???? ????? ???????. ????? ??? ??? ???????? ?????? ?????? (
Black Light
) ??
BL
??? ???????? ???????.
????? ????? ????? ??? ??????? ?? ????
???? ????? ???? ?????
.
??????? ????????
[
???
]
????? ?????? ??? ??????? ??
?????
??? ??? ????? ?? ??????? ??????? ????????? ????????? ???? ???? ??????
?????
?????? ???? ??????
???? ??? 99% ?? ??????? ???? ??? ??? ????? ???? ?? ?????? ???????
UVA
. (????? ??? ????? ???????? ???????? ?? ??????? ??? ??????? ???? ??? ????????? ???????? ?????? ????
???????
).
??????
??????? ????
????
????? ?????? ??????? ?? ??? ????????? ?????
????
??????? ???????
?????????
???????? ??
????????
???????? ??? ?????? ??? ????? ???????? ??? ???????
????????
.
????
???????
?????? ????? ????? ????? 90% ??
?????
?? ????? ?????? ??? 350 ???????? ???? ???? ????? 90% ??
?????
???? ??? ??300 ? ?.
[4]
[5]
[6]
?????? ????????
???? ?? 200 ???????? ???? ???? ??????? ???
??????
?????? ????
???????
??? ????? ??? 200 ???????? ???? ???? ??? ??????
????????
???????
???????
???? ????? ??????. ???
??????????
????? ???? ???? ??????? ?? ??? 150-200 ? ?. ???? ??????? ???? ?????? ??? ?????? ???????
?????? ????????
??????
??????
??? ??? ???? ??? ?? 200 ? ?. ??????? ?? ???? ???? ?? ????????? ??? ??????? ???? ?? ?? ?? ?? ???? ??? ???? ???????? ?????? ??????? ????? ?? ??????. ??? ??????? ??????? ??? ????? ???? ?????? ????? (circular dichroism spectrometer) ???? ?????????? ??? ???? ?? ??? ?????? ??????.
Extreme UV
??? ??????? ??? ??????? ?????? ?????? ???????? ??
??????
: ??????? ???? ???? ?? 30 ? ? ?????? ???????? ??
??????????? ?????????
?? ????????? ????
??????
(???? ??????? ???????)? ????? ??????? ???? ???? ?? ??? ?????? ??
???????????
???? ??????? ??????? ???
??????
????. ????? ????? ?????? ?????? ????? ??????? ??????
EUV/XUV
???? ?????? ???? ?????? ???????
????????
(
He
+
) ??? ????? 30.4 ? ?. ???? ?????? ???????? ???? ???? ??? ??????? ???? ???????? ????? ????? ????? ?????? ??????? ???? ????? 50% ?? ????? ???
??????
??? ??????? ?????? ??? ????? ???? ?????. ???
???????????
??????
???????????
?? ?????
??????? ??????
??? ?????? ??????? ????? ?????? ???? ??????????. ????? ??????? ??? ???????
???????????
????
??????? ????????
.
????? ??????? ??? ???????? ?????? ???????
[
???
]
????? ??????? ???? ??????
[
???
]
????? ???????? ??????? ?? ?????? ?????? ???????? ?? ??? ???????? (
UVB
) ??? ????? ??? ?????
??????? ??
??????
. ????????? ???? ?? ???? ????? ?????? ?? ????? ??????
????????
????? ??
???????? ???????
?????? ?? ???
??????? ??
??????
.
[7]
????? ??? ????? ???
??? ??????
(?? ?????? ??? ??????)? ????? ????
??????
?????? ?????. ????? ?????? ??? ???
?????????
???? ?? ????
???????
?? ??????
?????
?????? ?????.
[8]
????
?????
????? ???? ???????
???????? ??
??? ?????? ???? ??? ???
???????
?? ??????? ???? ???????
??????
???? ?? ?????? ?? (
UVB
) ???? ?? ????? ??? ???????
??????
?????
???? ?????? ????? ???????.
[8]
?????????
[
???
]
??? ??????? ?? ?????? ???????? (UVB) ???? ??? ???????? ??. ???????? ??? ????
??? ????? ????? ??????
????? ??????. ??? ?????? ???????? ?? ?????? ?????? ???????? (????? ????? ???
??? ??????
) ???? ???? ??????? ??????? ????? ??? ????? ??????. ?????? ????? ????? ????? ????? ???. ???????? ?????? ????? ?????? ??????? ??? ???? ??? ??? ???? ?? ???? ????. ???????? ???? ???? ??? ??? ????? ??? ?????? ???????? ?????? ??? ???? ????? ???? ???? ??? ??????? ????? ??? ????????? ??????? (
UVA
). ??? ?????? ??????? ??????? ???????? ?????? ???? ?? ?????? ?????? ??? ?????????.
??????? ????
[
???
]
?????? ??? ????????? ????????? ????? ??????
??????? ???????
???
???????
???????
. ?????? ????? ??? ????????? ??????? (
UVA
) ?? ????? ????????? ??????? psoralens ????? ?? ???? ???? ???? (PUVA treatment). ??? ???????? (
UVB
) ??????? ?? ?????? ?? ??? ??????.
???? ??????? ???? ??????
[
???
]
{????? ??????
???? ?????
}
?????? ?????? ????? ????? ??????? ??? ????????? ???????? (UVB) ?? ????? ????? ????? ???? ????? ????? ?????. ??????? ?????? ??? ?????? ????? ????? ????? ????? ??? ????????? ?? ???? ??? ??????? ???? ????? ??????
??????
??????
??????? ???????
?????.
[9]
???????
??? ???????? ????????
????? ????? ???? ??? ????? ????? ?????? (???? ??? ????? ???????)? ????? ?? ??? ??? ?? ???? ???? ????? ?????? ?????? ??? ????????? ?? 92% ?? ??? ??????????.
[10]
?????
UVC
?? ?????? ???? ?? ??? ?????? ?? ?????? ??? ????????? ??????? ????? ????? ???? ??? ???? ???
???? ????? ?????
? ?? ??? ????????? ?????? ???? ???
????? ?????
?????? ?????? ??????? ?? ???? ????? ?????? ??? ??? ?? ?????
???????
???? ??? ???? ??????? ???????.
??????? ??? ?????
[
???
]
≪?????? ????? ??? ??????? ???? ???? ??
???
?????
????? ?????
???? ?????
?????
. ??????? ????? ???? ???????? ?????? ?? ??? ????? ?? ?????? ?????? ????????? ??? ????? ?????? ????? ???????? ???????? ??????? ??????? ???
?????
???? ?????? ?????? ????????? ????? ???? ?????
??????
???? ???????
?????
. ≫ ? Matsumura and Ananthaswamy? 2004
[11]
?????? ????? ??? ???????? ??????
UVA
????????
UVB
???????
UVC
?? ???? ?????
??????
?????????
???????? ????
???????
?????
. ?????? ???????
UVA
?????????
UVB
??????? ?????
??????? ?
???????
??????
.
[12]
????
???????
???????
UVA
? ??? ??? ???? ????? ?? ?????? ????? ????? ?????? ?????? ????? ???? ????? ????
??????
?????
???? ????? ??? ?????
????? ??????
DNA. ??? ????? ???? ????? ?? ???? ???? ????? ??? ?????? ?????? ??? ???? ??? ???? ??? ???? ?????? ?????? (sunscreen) ????? ?? ???????? ??????? UVB ????. ???? ???? ?? ???? ????? ?????? ???? ????? ??????? ???????? ???????? ?????? ?????? ????? ??? ??????? ???? ???????? ??? ????
?????????
????????? ????? ????? ?????? ?????? ??? ??????. ??? ???? ??? ??????? ????? ??? ????? ????? ??????? ???? ????? ??????? ???????? ??????? ???? ??? ???? ?????? ???? ?????? ?? ????????.
[13]
??? ?????? ????????
UVB
??? ????? ??????? ????? ????? ?????
?????????
???? ?????? ???? ??
UVA
? ?? ???? ??????? ??????? ????? ??????. ???????? ????
??????
????? ?????? ?? ????? ?????? ?????
?????? ???????
???? ????? ?? ??? ?????
?????????
??????
????? ??????
. ?????? ????
??? ?????????
????? ?? ????? ??? ????? ?? ???? ?? ??? ????? ???? ?????? ???????? ? "AA" ???? ???????? ??????? "CC"? ????? ?????? ????? ?????
????? ????????
????????
???
????? ?????????
??? ???? ??? ???? ?? DNA ?????
???????
????? ??????? ????? ????????. ??? ????
?????? ???????
??? ???? ?????? ????????? ???
?????? ???????
??????? ?? ?????? ????? ??????? ?? ??????? ????????
??????
?????????
? ??? ?????? ???????? ????? ?? ??????? ???? ???? ???????? ??? ????
??? ???????
.
???????? ??????? ????? ?????? ???? ????? ??? ???????? ??? ????? ?????? ????? ?? ?????
UVA
(??? ??? ?????) ????? ?????? ?????? ????? ?????
UVB
???? ????? ????. ??? ???????? ???? ?????? ????? ??????? ??? ???? ??????? ????? ??????
?????
???????. ???? ???????? ????? ??????? ??????? ?????? ?????? ?? ????
?????
??? ????
??????
????? ??????? ????? ??????? ????? ??? ???? ???? ?????? ?????? ????? ?? ????? ??? ?????? ??????? ?? ???? ??
UVB
???????? ?? ????
?????
????? ?? ?????? ??????? ??
?? UVA
??? ?????? ?????? ?????? ???? ???????? ???????? ????? ????? ????? ?????? ???
???? ????? ??????????
???? ?????? ?????? ?????? ??????? ????? ???????
UVA
????? ???????? ??????
??????
??????? ?? ?????? ????? ??????? ????? ????????? (Phlebodium aureum).
?????
[
???
]
?????? ????? ?????? ????????
UVB
?? ????? ?????? ??????? ?? ????
?????
?????
???? ?????
? ??????? ???????
??????
??????? ?????? ????? ??????? ??????? ????? ?? ???????
???????
????? ??????? ????
??????
???????
UVC
? ??????
??????
???? ???? ?????? ????? ??????? ?? ??????? ???????? ???? ???? ??? ?????? ????? (??? ???
?????????
) ???? ???? ??? ?????????? ????? ??????
??????
???.
??????
???????? ??????? ???? ????? ?????? ??? ????
???????
?????????
??????? ???? ?? ????????? ?????? ??? ?????? ????? ??
??????
?? ????? ????? ??????? ???????
UVA
??? ?????
?????????
??????????
????? ??????
???????
?? ???? ??? ????
???????
?????????
???
?????? ????????
(??? ???? ??? ??? ??? ??????
???????
) ???? ???? ?????? ????? ???????.
?????
???????
????? ??????? ?? ??????? ???? ??? ??? ??????? ?? ?????? ????? ????? ?????? ????? ??????? ?? ?????.
??????? ???? ?? ??????? ????? ??????
[
???
]
??????
????????
?????????
?????????
??????????? ????? ?? ??????? ????? ??????? ?????? ??? ?????? ???? ??????? ??????? ???????? ???
??????? ????????
???
?????? ????????
??????? ??????
???????? ?????????? ???????? ???
????????
? ?????? ?????? ????? ?????? ???? ??? ???????
??????
? ???????? ??? ?????? ??
???????
????????
???? ???? ??? ?????? ?????? ?????? ????? ???? ??????? ?????????? ???? ??? ???? ?????? ??????? ??
?????
????????.
?????? ??????? ??????????
[
???
]
????????
????????? ?????? ????? ??????? ?????? ???????
???????
????????
?????????
?????? ???? ????
??????
?? ?? ???? ? (??????? ??????) ???? ???????? ????? ?? ??? ??????? ????? ???????? ?????? ????? ??????
?????
???? ?? ?????? ?????? ????????? ???? ?????? ???? ????? ???? ??????. ???? ?????? ???????
???????? ???????
???? ???? ??????
UVA
?
UVB
??? ???????? ??????? ?????????????? ??? ?????? ?? ??????? ?????? ????? ??????? ???
???? ????? ??????????
??????
?????
.
??????? ?????? ????? ???????
[
???
]
????? ??????
[
???
]
????? ??????
?? ??? ???? ??????
UVA
?????? ??
?????
??????. ????? ????????? ??????? ?? ??? ????????? ??????? ????? ?? ???????? ?? ???
???????
???????
???? ????
????
???? ???
????????
????? ?????? ?????? ??????? ??? ??? ??? ??????? ???????? ?????? (
?????? ????
???? ?????
??????? ??????) ???? ???????? ???? ????? ????? ???????? ??????? ?????? ???????? ??????? ??? ??????? ??????? ??????? ?????? (?????? ???????? ???????) Hologram
??????
??? ????? ????? ??? ???????.
??????? ???????
???? ???? ??? ?????? ????? ???? ????? ??????? ????? ???????.
?????? ?????????
[
???
]
???? ???
????????
????? ??? ?????? ??????
????
????
??????
??? ????
?????
? ????? ?????
???????
???? ???? ????? ??????? ?? ??????? ??????? ??? ??????? ?????? ??? ??? ????. ????? ?????? ????????? ?????? ???????? ???? ???? ????? ??????. ??? ?? ??????? ???? ??????? ?? ????? ?????? ??? ????? ????? ??????? ???? ?? ????? ??? ???????? ??????.
?????? ?????? ????? ???? ??????? ???????? ??????? ?????? ????? ?????? ?? ?????
???????
????? ?????????? ????? ????? - ????? ?????? ????? ???????? ?????? ??????
??????
-
???????
????????.
?????? ???????
[
???
]
???? ??????? ??????? ??????? ?? ???? ????? ??? ??????. ???? ???? ??????? ???? ?????? ?? ??? ????????? ?? ?????? ????? ??? ????? ???? ???.
????? ???? ???? ?????? ?????? ?????? ?????? ?????? ?? ??????? ??????? ???? ?????? ??? ????? ????? ?????? ????? ??????? ???????? ??? ?????? ???? ??????.
????? ????? ??????
[
???
]
??? ???????? ??????? ????? ?????? ?????? ?????? ?????? ???? ??
??? ????????
??????
?????? ?????????
?????
??????? ?????????
? ??????? ????? ?????? ?????? ?? ???? ????? ?????? ?????? ???? ???? ?????? ???? ??????? ????? ??????????.
????? ???????
[
???
]
????? ????? ?????? ?????? ?????? ???????
???????? ???????
??????? ????? ???????? ?? ????? ???????? ???. ?????? ????? ??? ???? ??? ????? ?????? ???? ???? ?????? ????? ??? ????? ??????? ?? ??? ????? ?? ??? ????? ?????? ?????? ???????.
???????? ?????????? ?????????
[
???
]
????? ????????? ????? ??????? ??? ????????? ????? ???
???????? ???????
??????? ????????
? ????? ?????? ??????? ??????
Green Fluorescent Protein GFP
??? ??????
???? ???????
?????? ????????? ??????????? ????? ????? ??????? ?????? ?????? ????? ??????? ??? ??? ???? ??? ????? ????????? ??????? ????????? ??? ???????.
???? ????????? ??????
[
???
]
??? ???? ????? ????? ???????? ????? ?????? ????? ??????? ?? ????? ????? ????? ?????? ???? ???????? ??????? ????????? ????? ??? ???????
PUVA
? ???? ?????? ????? ?????? ???????? ???
?????
.
???? ?????
[
???
]
???????
[
???
]
|
---|
- UVA
: 400?315
nm
- UVB
: 315?290 nm
- ????? ???????? ?? ?? ????? ???? ???
|
??????? UVA
| |
---|
??????? UVB
| |
---|
??????? UVA+UVB
| |
---|
?????? ????????
|
- ??????? ?????? ???????? ?????? ?????????? (IUPAC)
:
UT07492
|
---|
????????? ??????
| |
---|
???????? ????????
| |
---|